Cat: MF-1001
IL1-beta, Mouse, HEK293 Cells,Tag Free: Product Information
> 95%, determined by SDS-PAGE
<0.010 EU per 1 ug of the protein by the LAL method.
Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells.
The EC50 for this effect is 1-4 pg/mL.
NP_032387
Human embryonic kidney cell, HEK293-derived mouse IL-1 beta/IL-1F2 protein
Val118-Ser269
17.5 kDa
Solution protein
Dissolved in sterile PBS buffer to a concentration of 0.2 mg/mL.
This solution can be diluted into other aqueous buffers. Centrifuge the vial prior to opening.
Avoid repeated freeze-thaw cycles.
It is recommended that the protein be aliquoted for optimal storage.
12 months from date of receipt, -20 to -70 °C as supplied.
Shipping with dry ice.
IL1-beta, Mouse, HEK293 Cells,Tag Free: Product Information
2 ug/lane protein was resolved with SDS-PAGE under non-reducing (NR) and reducing (R) conditions and visualized by CoomassieBlue staining.
Size-exclusion chromatography of recombinant mouse IL1b protein (280 nm absorbance)
Recombinant mouse IL1b (Catalog # MF-1001B) stimulates cell proliferation of D10.G4.1 mouse helper T cells.
IL1-beta, Mouse, HEK293 Cells,Tag Free: Product Information
IL1 beta; IL-1; IL1B; IL-1b; IL1-BETA; IL-1F2; IL1F2IL-1 beta; interleukin 1-beta
IL1-beta, Mouse, HEK293 Cells,Tag Free: Product Information
Interleukin 1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 17% amino acid (aa) identity in mouse. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects (1-4). The mouse IL-1 beta cDNA encodes a 269 aa precursor. A 117 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine (5, 6). The 17 kDa mature mouse IL-1 beta shares 90% aa sequence identity with cotton rat and rat and 65%-78% identity with canine, equine, feline, human, porcine, and rhesus IL-1 beta.
1. Allan, S.M. et al. (2005) Nat. Rev. Immunol. 5:629.
2. Boraschi, D. and A. Tagliabue (2006) Vitam. Horm. 74:229.
3. Kornman, K.S. (2006) Am. J. Clin. Nutr. 83:475S.
4. Isoda, K. and F. Ohsuzu (2006) J. Atheroscler. Thromb. 13:21.
5. Gray, P.W. et al. (1986) J. Immunol. 137:3644.
6. Martinon, F. and J. Tschopp (2007) Cell Death Differ. 14:10.
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