Cat:
, , ,: Product Information
> 95%, determined by SDS-PAGE
<0.010 EU per 1 ug of the protein by the LAL method.
Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells.
The EC50 for this effect is 0.5-3 ng/mL.
NP_001165097
Human embryonic kidney cell, HEK293-derived human VEGF protein
Ala27-Arg191
19.2 kDa(Monomer)
Disulfide-linked homodimer
Solution protein
Dissolved in sterile PBS buffer to a concentration of 0.2 mg/mL.
This solution can be diluted into other aqueous buffers. Centrifuge the vial prior to opening.
Avoid repeated freeze-thaw cycles.
It is recommended that the protein be aliquoted for optimal storage.
12 months from date of receipt, -20 to -70 °C as supplied.
Shipping with dry ice.
, , ,:SDS-PAGE & Bioactivity
4 ug/lane protein wasresolved with SDS-PAGE under non-reducing (NR) and reducing (R) conditionsand visualized by CoomassieBlue staining.
Size-exclusion chromatography of recombinant human VEGF165 protein (280 nm absorbance)
Recombinant human VEGF165 (Catalog # HF-2020) stimulates cell proliferation of the HUVEC human umbilical vein endothelial cells.
, , ,:Synonyms
, , ,:Background
